The aim of the deliverable is to produce a manuscript on the key component “Comparative Biodiversity Trend Analyses”. This is a crosscutting key component that links WP4, 5, and 6, and has the main aim to assess current (WP4) and future distribution patterns of freshwater biodiversity under climate change (WP5) and other stressors (WP6) at the basin scale. There are three target basins in this key component that have been investigated extensively: the Ebro, the Elbe, and the Danube. In this Deliverable, we have applied climate envelope models and other statistical tools to forecast current and future distributions of the brown trout, Salmo trutta. We decided to focus on this emblematic species instead of other because (1) it is a native species widely distributed across Europe, (2) it has a high occurrence in the three target basins, (2) it is considered very sensitive to environmental variables related to climate or other stressors, (3) it has a high recreational and commercial value and thus its conservation and management is a priority, and (4) there is available and easily accessible data gathered with similar methods. In comparison to previous Salmo trutta forecasts that only focus on climatic variables, our manuscript examined the predictive role of multiple drivers, including climate, land uses and stream topography. The manuscript was published in the Diversity and Distributions journal in 2013 (link to article), which has an Impact Factor of 6.122. It includes 8 authors from 5 different BioFresh partners (University of Barcelona, Leibniz- Institute of Freshwater Ecology and Inland Fisheries, University of Natural Resources and Life Sciences, Museum National d’Histoire Naturelle, Royal Belgian Institute of Natural Sciences).